a+m Proteome Science was founded in 2002 by neuroscientists of the Charité University Hospital in Berlin. Our goal is to perform sensitive and reproducible quantitative proteome analyses.
The basic problem with quantifying proteome research was and is the fact that the results are poorly reproducible. Premature application of methods that had not been adequately tried and proven resulted in a loss of resources and a loss of confidence in this area of research.
We therefore began by investing our resources in methodological studies and worked on improving the technique of 2-DE gel electrophoresis for six years.
Today we are able to produce up to 24 two-dimensional gels in a single experimental design and thus to carry out biometrically reliable comparisons between groups with large enough sample sizes. These gels can be excellently reproduced.
We have developed strategies for conducting quantitative analysis of approx. 5000 protein spots from homogenates and subcellular fractions of rat brain. We are thus able to visualize numerous low-abundant proteins and to conduct function-specific proteome analysis of the brain. However, our method can also be used to analyze most other, less complex tissues.
Due to the excellent reproducibility of our gels we can detect 25% differences in spot intensity of 50% to 80% of all matched spots.
This increased statistical power enables us to search for biomarkers for specific diseases and candidate proteins for the development of new drugs.
We are now using this range of methods in our own research projects and also offering them as services to others.
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Rat brain, amygdala, homogenate, pH 4-7, 1224 protein spots |
Cancer stem cell line (melanoma), pH 4-7, 1264 protein spots |
Quantify proteomes?
Of course we can!